recombinant anti hmgb1 polyclonal antibody  (Bio X Cell)

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Primer sequences for quantitative polymerase chain reaction analysis.

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Expression of (A) HMGB1, (B) TGF-β1 and (C) NF-κB in the renal tissue of patients in the CAN group. Protein was stained as brown. Magnification, ×100. HMGB1, high mobility group protein B1; TGF-mo transforming growth factor-B1 NF-κB, nuclear factor-κB; CAN, chronic allograft nephropathy.

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Expressing, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Expression of HMGB1, TGF-β1 and NF-κB in the CAN and control groups.

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Western blot analysis of HMGB1, TGF-β1 and NF-κB. (A) Immunobloting of HMGB1, TGF-β1 and NF-κB proteins, with β-actin as the reference gene. Expression levels of (B) HMGB1 (C) TGF-β1 and (D) NF-κB in renal tissues. *P<0.05 vs. control; #P<0.05 vs. CAN grade I; &P<0.05 vs. CAN grade II. HMGB1, high mobility group protein B1; TGF-β1, transforming growth factor-β1; NF-κB, nuclear factor-κB; CAN, chronic allograft nephropathy.

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Western Blot, Gene Expression, Control

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction analysis of (A) HMGB1 (B) TGF-β1 and (C) NF-κB mRNA expression levels in renal tissues. *P<0.05 vs. control; #P<0.05 vs. CAN grade I; &P<0.05 vs. CAN grade II. HMGB1, high mobility group protein B1; TGF-β1, transforming growth factor-β1; NF-κB, nuclear factor-κB; CAN, chronic allograft nephropathy.

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Relative expression of HMGB1, TGF-β1 and NF-κB mRNA in the CAN and control groups.

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Pearson correlation analysis of the association between the protein and mRNA expression levels of HMGB1, TGF-β1 and NF-κB (n=57).

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy

doi: 10.3892/etm.2017.5319

Figure Lengend Snippet: Spearman correlation analysis of the association between the protein and mRNA expression levels of HMGB1, TGF-β1 and NF-κB and CAN grade (n=57).

Article Snippet: Polyclonal antibody rabbit anti-human HMGB1 (#BA2361; 1:200), TGF-β1 (#BM4876; 1:200) NF-κB p65 (#BM3946; 1:200), and goat anti-rabbit IgG conjugated to horseradish peroxidase (#BM3895; 1:1,000) were purchased from Wuhan Boster Biological Engineering Co., Ltd.

Techniques: Expressing